Journal article
Antibody to Plasmodium falciparum Variant Surface Antigens, var Gene Transcription, and ABO Blood Group in Children with Severe or Uncomplicated Malaria
P Barua, MF Duffy, L Manning, M Laman, TME Davis, I Mueller, A Haghiri, JA Simpson, JG Beeson, SJ Rogerson
Journal of Infectious Diseases | OXFORD UNIV PRESS INC | Published : 2023
Abstract
Background: Antibodies to variant surface antigens (VSAs) such as Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1) may vary with malaria severity. The influence of ABO blood group on antibody development is not understood. Methods: Immunoglobulin G antibodies to VSAs in Papua New Guinean children with severe (n = 41) or uncomplicated (n = 30) malaria were measured by flow cytometry using homologous P falciparum isolates. Isolates were incubated with ABO-matched homologous and heterologous acute and convalescent plasma. RNA was used to assess var gene transcription. Results: Antibodies to homologous, but not heterologous, isolates were boosted in convalescence. The relationship b..
View full abstractRelated Projects (2)
Grants
Awarded by University of Melbourne
Funding Acknowledgements
Laboratory work was supported by funds from the National Health and Medical Research Council (NHMRC) of Australia (grant numbers 1092789 and 513782). This work is supported in part by the Australian Centre for Research Excellence in Malaria Elimination, funded by the NHMRC (grant number 1134989). J. A. S. and J. G. B. are funded by NHMRC investigator grants (grant numbers 1196068 and 1173046). P. B. was supported by a University of Melbourne International Research Scholarship